{"100661":{"#nid":"100661","#data":{"type":"news","title":"Ying Shen publishes video article in Journal of Visualized Experiments","body":[{"value":"\u003Cp\u003ESchool of Biology PhD candidate Ying Shen (video actor) and her PI Francesca Storici (video director) present concepts and methods of using RNA-containing oligonucleotides (oligos) for studying RNA-driven genetic changes in the video\/article \u0027\u003Ca href=\u0022http:\/\/www.jove.com\/Details.stp?id=2152\u0022\u003EGeneration of RNA\/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides\u003C\/a\u003E\u0027 published in the Journal of Visualized Experiments. Click on the title link for full video and article.\n\u003C\/p\u003E\n\u003Cp\u003EResults are obtained that reveal generation of an RNA\/DNA hybrid in yeast chromosomal DNA based on the transfer of genetic information from the RNA tract of the oligo to a mutated marker gene present in the yeast genome.  As shown in this video, transformation using RNA-containing oligos requires essentially only a few additional steps compared to transformation using DNA molecules, to prevent degradation by RNases. Thus, transformation using RNA-containing oligos is not limited to the yeast system, but can be applied to any organism or cell type where transformation by DNA oligos is proficient.\n\u003C\/p\u003E\n\u003Cp\u003EWhile several \u003Cem\u003Ein vitro\u003C\/em\u003E studies have been conducted to characterize the function of factors that can recognize RNA in a hybrid with DNA, the identity and the \u003Cem\u003Ein vivo\u003C\/em\u003E functions of factors that could affect RNA\/DNA hybrid stability remain mostly unknown. The possibility to utilize RNA-containing oligos of a significant length (50 to 80-mers) and optimal quality (such as Thermo Scientific Dharmacon RNA-containing oligos) has opened the way to examining a wide range of molecular processes directly \u003Cem\u003Ein vivo\u003C\/em\u003E in the cells of interest. As shown in this video, targeting cells by RNA-containing oligos provides the opportunity to embed ribonucleotides into genomic DNA in cells. The stability, function and consequences of these \u003Cem\u003Ein vivo\u003C\/em\u003E generated RNA\/DNA hybrids can be analyzed and characterized, potentially uncovering unknown mechanisms of RNA-driven DNA editing and modification.  \n\u003C\/p\u003E\n\u003Cp\u003EThe work was supported by the Georgia Cancer Coalition grant-R9028.\u003Cbr \/\u003E\nThe video production for this article was sponsored by Thermo Fisher Scientific.\n\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":[{"value":"Grad student Ying Shen publishes video article in Journal of Visualized Experiments"}],"field_summary":[{"value":"School of Biology PhD candidate Ying Shen (video actor) and her PI Francesca Storici (video director) present concepts and methods of using RNA-containing oligonucleotides (oligos) for studying RNA-driven genetic changes in the video\/article \u0027\u003Ca href=\u0022http:\/\/www.jove.com\/Details.stp?id=2152\u0022\u003EGeneration of RNA\/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides\u003C\/a\u003E\u0027 published in the Journal of Visualized Experiments. Click on the title link for full video and article.","format":"limited_html"}],"field_summary_sentence":[{"value":"Ying Shen publishes video article in Journal of Visualized Experiments"}],"uid":"27245","created_gmt":"2011-01-04 01:00:00","changed_gmt":"2016-10-08 03:11:09","author":"Troy Hilley","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2011-01-04T00:00:00-05:00","iso_date":"2011-01-04T00:00:00-05:00","tz":"America\/New_York"},"extras":[],"hg_media":{"100671":{"id":"100671","type":"image","title":"Ying Shen","body":null,"created":"1449178159","gmt_created":"2015-12-03 21:29:19","changed":"1475894717","gmt_changed":"2016-10-08 02:45:17"}},"media_ids":["100671"],"related_links":[{"url":"http:\/\/www.biology.gatech.edu\/people\/francesca-storici","title":"Francesca Storici"},{"url":"http:\/\/www.biology.gatech.edu\/","title":"School of Biology"}],"groups":[{"id":"1275","name":"School of Biological Sciences"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003ESchool of Biology\u003C\/strong\u003E\u003Cbr \/\u003EBiology\u003Cbr \/\u003E\u003Ca href=\u0022mailto:admin@biology.gatech.edu\u0022\u003EContact School of Biology\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-3700\u003C\/strong\u003E","format":"limited_html"}],"email":["admin@biology.gatech.edu"],"slides":[],"orientation":[],"userdata":""}}}