{"173791":{"#nid":"173791","#data":{"type":"event","title":"Yunfeng Chen - Ph.D. Proposal Presentation","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor\u003C\/strong\u003E: Dr. Cheng Zhu (BioE, Gatech)\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECommittee\u003C\/strong\u003E:\u0026nbsp; Dr. Larry McIntire (BioE, Gatech), Dr. Wilbur Lam (BioE, Gatech), Dr. Xiaoping Du(University of Illinois at Chicago) and Dr. Renhao Li (Emory University)\u003Cbr \/\u003EIn hemostasis, platelet adhesion and signaling play key roles. Specifically, two platelet receptors: GPIb\u03b1 and GPIIb\/IIIa mediate the early and mid-stages of platelet adhesion. Platelet glycoprotein Ib\u03b1 (GPIb\u03b1) is part of the GPIb\u03b1-V-IX complex that constitutes the receptor for von Willebrand factor (VWF). Its binding to VWF A1 domain enables rolling of platelets on the sites of vascular injury. GPIIb\/IIIa is also known as integrin \u03b1IIb\u03b23 and is found exclusively on platelets; upon activation, it allows for platelet stable adhesion to VWF on the surface of sub-endothelium and promotes the expansion of the platelets plug by cross-linking via Arg-Gly-Asp (RGD) bearing ligands like soluble fibrinogen and VWF. Ligation of GPIb\u03b1 and GPIIb\/IIIa has been reported to trigger outside-in platelet-activating signals in a sequential fashion. In this proposal, I will study GPIb\u03b1 and GPIIb\/IIIa in the context of platelet adhesion and signal initiation. The goal is to address how ligand binding of GPIb\u03b1 and GPIIb\/IIIa initiates signaling in a mechanically dependent way and how GPIb\u03b1 and GPIIb\/IIIa trigger platelet activation. I propose to conduct single-molecular level experiments with a fluorescence biomembrane force probe (fBFP) to systematically correlate mechanically dependent GPIb\u03b1 and GPIIb\/IIIa ligation with readouts of platelet activation including GPIIb\/IIIa up-regulation and unbending conformational change, platelet intracellular Ca\u003Csup\u003E2+\u003C\/sup\u003E signal and lipid scrambling on the plasma membrane.\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003E\u0022Mechanical Triggering of Platelets by GPIb\u03b1 and GPIIb\/IIIa\u0022\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Mechanical Triggering of Platelets by GPIb\u03b1 and GPIIb\/IIIa"}],"uid":"27547","created_gmt":"2012-11-28 14:47:23","changed_gmt":"2016-10-08 02:01:26","author":"Chris Ruffin","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2012-12-11T14:30:00-05:00","event_time_end":"2012-12-11T16:30:00-05:00","event_time_end_last":"2012-12-11T16:30:00-05:00","gmt_time_start":"2012-12-11 19:30:00","gmt_time_end":"2012-12-11 21:30:00","gmt_time_end_last":"2012-12-11 21:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"569","name":"bioengineering"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:chris.ruffin@ibb.gatech.edu\u0022\u003EMr. Christopher Ruffin\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}