{"235771":{"#nid":"235771","#data":{"type":"news","title":"Unusual Mechanism of DNA Synthesis Could Explain Genetic Mutations","body":[{"value":"\u003Cp\u003EResearchers have discovered the details of how cells repair breaks in both strands of DNA, a potentially devastating kind of DNA damage.\u003C\/p\u003E\u003Cp\u003EWhen chromosomes experience double-strand breaks due to oxidation, ionizing radiation, replication errors and certain metabolic products, cells utilize their genetically similar chromosomes to patch the gaps via a mechanism that involves both ends of the broken molecules. To repair a broken chromosome that lost one end, a unique configuration of the DNA replication machinery is deployed as a desperation strategy to allow cells to survive, the researchers discovered.\u003C\/p\u003E\u003Cp\u003EThe collaborative work of graduate students working under Anna Malkova, associate professor of biology at Indiana University-Purdue University Indianapolis (IUPUI) and \u003Ca href=\u0022http:\/\/www.biology.gatech.edu\/people\/kirill-lobachev\u0022\u003EKirill Lobachev\u003C\/a\u003E, associate professor of \u003Ca href=\u0022http:\/\/www.biology.gatech.edu\/index.php\u0022\u003Ebiology\u003C\/a\u003E at the Georgia Institute of Technology, was critical in the advancement of the project. The group\u2019s research was scheduled to be published Sept. 11 in the online edition of the journal \u003Cem\u003ENature\u003C\/em\u003E, with two graduate students, Sreejith Ramakrishnan of IUPUI, and Natalie Saini of Georgia Tech, as first authors. Other collaborators include James Haber of Brandeis University and Grzegorz Ira of the Baylor College of Medicine.\u003C\/p\u003E\u003Cp\u003E\u201cPreviously we have shown that the rate of mutations introduced by break-induced replication is 1,000 times higher as compared to the normal way that DNA is made naturally, but we never understood why,\u201d Malkova said.\u003C\/p\u003E\u003Cp\u003ELobachev\u2019s lab used cutting-edge analysis techniques and equipment available at only a handful of labs around the world. This allowed the researchers to see inside yeast cells and freeze the break-induced DNA repair process at different times. They found that this mode of DNA repair doesn\u2019t rely on the traditional replication fork \u2014 a Y-shaped region of a replicating DNA molecule \u2014 but instead uses a bubble-like structure to synthesize long stretches of missing DNA. This bubble structure copies DNA in a manner not seen before in eukaryotic cells.\u003C\/p\u003E\u003Cp\u003ETraditional DNA synthesis, performed during the S-phase of the cell cycle, is done in semi-conservative manner as shown by Matthew Meselson and Franklin Stahl in 1958 shortly after the discovery of the DNA structure. They found that two new double helices of DNA are produced from a single DNA double helix, with each new double helix containing one original strand of DNA and one new strand.\u003C\/p\u003E\u003Cp\u003E\u201cWe demonstrated that break-induced replication differs from S-phase DNA replication as it is carried out by a migrating bubble instead of a normal replication fork and leads to conservative DNA synthesis promoting highly increased mutagenesis,\u201d Malkova said.\u003C\/p\u003E\u003Cp\u003EThis desperation replication triggers \u201cbursts of genetic instability\u201d and could be a contributing factor in tumor formation.\u003C\/p\u003E\u003Cp\u003E\u201cFrom the point of view of the cell, the whole idea is to survive, and this is a way for them to survive a potentially lethal event, but it comes at a cost,\u201d Lobachev said. \u201cPotentially, it\u2019s a textbook discovery.\u201d\u003C\/p\u003E\u003Cp\u003EDuring break-induced replication, one broken end of DNA is paired with an identical DNA sequence on its partner chromosome. Replication that proceeds in an unusual bubble-like mode then copies hundreds of kilobases of DNA from the donor DNA through the telomere at the ends of chromosomes.\u003C\/p\u003E\u003Cp\u003E\u201cSurprisingly, this is a way of synthesizing DNA in a very robust manner,\u201d Saini said. \u201cThe synthesis can take place and cover the whole arm of the chromosome, so it\u2019s not just some short patches of synthesis.\u201d\u003C\/p\u003E\u003Cp\u003EThe bubble-like mode of DNA replication can operate in non-dividing cells, which is the state of most of the body\u2019s cells, making this kind of replication a potential route for cancer formation.\u003C\/p\u003E\u003Cp\u003E\u201cImportantly, the break-induced replication bubble has a long tail of single-stranded DNA, which promotes mutations,\u201d Ramakrishnan said.\u003C\/p\u003E\u003Cp\u003EThe single-stranded tail might be responsible for the high mutation-rate because it can accumulate mutations by escaping the other repair mechanisms that quickly detect and correct errors in DNA synthesis.\u003C\/p\u003E\u003Cp\u003E\u201cWhen it comes to cancer, other diseases and even evolution, what seems to be happening are bursts of instability, and the mechanisms promoting such bursts were unclear,\u201d Malkova said.\u003C\/p\u003E\u003Cp\u003EThe molecular mechanism of break-induced replication unraveled by the new study provides one explanation for the generation of mutations.\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EThis research is supported by the National Institutes of Health under awards RO1GM082950, RO1GM084242, RO3ES016434, GM76020, and by the National Science Foundation under award MCB-0818122. Any conclusions or opinions are those of the authors and do not necessarily represent the official views of the NIH or NSF.\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECITATION\u003C\/strong\u003E: N. Saini, et al., \u201cMigrating bubble during break-induced replication drives conservative DNA synthesis,\u201d (Nature, 2013). \u003Ca href=\u0022http:\/\/dx.doi.org\/10.1038\/nature12584\u0022 title=\u0022http:\/\/dx.doi.org\/10.1038\/nature12584\u0022\u003Ehttp:\/\/dx.doi.org\/10.1038\/nature12584\u003C\/a\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EResearch News\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EGeorgia Institute of Technology\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003E177 North Avenue\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EAtlanta, Georgia \u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EMedia Relations Contacts:\u003C\/strong\u003E \u003Cbr \/\u003EGeorgia Tech: Brett Israel (404-385-1933) (\u003Ca href=\u0022mailto:brett.israel@comm.gatech.edu\u0022\u003Ebrett.israel@comm.gatech.edu\u003C\/a\u003E) or John Toon (404-894-6986) (\u003Ca href=\u0022mailto:jtoon@gatech.edu\u0022\u003Ejtoon@gatech.edu\u003C\/a\u003E) .\u003C\/p\u003E\u003Cp\u003EIUPUI: Rich Schneider (317-278-4564) (\u003Ca href=\u0022mailto:rcschnei@iu.edu\u0022\u003Ercschnei@iu.edu\u003C\/a\u003E).\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EWriter\u003C\/strong\u003E: Brett Israel\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EResearchers have discovered the details of how cells repair breaks in both strands of DNA, a potentially devastating kind of DNA damage.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Researchers have discovered how cells repair a potentially devastating kind of DNA damage."}],"uid":"27902","created_gmt":"2013-09-10 10:21:16","changed_gmt":"2016-10-08 03:14:53","author":"Brett Israel","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2013-09-11T00:00:00-04:00","iso_date":"2013-09-11T00:00:00-04:00","tz":"America\/New_York"},"extras":[],"hg_media":{"235751":{"id":"235751","type":"image","title":"Exploring DNA repair","body":null,"created":"1449243659","gmt_created":"2015-12-04 15:40:59","changed":"1475894911","gmt_changed":"2016-10-08 02:48:31","alt":"Exploring DNA repair","file":{"fid":"197664","name":"lobachev-saini.jpg","image_path":"\/sites\/default\/files\/images\/lobachev-saini_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/lobachev-saini_0.jpg","mime":"image\/jpeg","size":563363,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/lobachev-saini_0.jpg?itok=EHhytsns"}}},"media_ids":["235751"],"groups":[{"id":"1188","name":"Research Horizons"}],"categories":[{"id":"140","name":"Cancer Research"},{"id":"146","name":"Life Sciences and Biology"}],"keywords":[{"id":"919","name":"Biochemistry"},{"id":"73441","name":"break-induced replication"},{"id":"73431","name":"cell division"},{"id":"2638","name":"DNA repair"},{"id":"73421","name":"dna synthesis"},{"id":"5718","name":"Genetics"},{"id":"8668","name":"Kirill Lobachev"},{"id":"6555","name":"molecular biology"},{"id":"68181","name":"Natalie Saini"}],"core_research_areas":[{"id":"39441","name":"Bioengineering and Bioscience"}],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EBrett Israel\u003C\/p\u003E\u003Cp\u003EResearch News\u003C\/p\u003E\u003Cp\u003E404-385-1933\u003C\/p\u003E\u003Cp\u003E\u003Ca href=\u0022mailto:brett.israel@comm.gatech.edu\u0022\u003Ebrett.israel@comm.gatech.edu\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":["brett.israel@comm.gatech.edu"],"slides":[],"orientation":[],"userdata":""}}}