{"255661":{"#nid":"255661","#data":{"type":"event","title":"PhD Proposal - Kipp Schoenwald","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EA Soft Fluidic Force Microscope Probe for Cellular Force Measurement\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003ECommittee:\u003Cbr \/\u003E\u003Cstrong\u003ETodd Sulchek, Ph.D. (Chair and PI), ME\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EOliver Brand, Ph.D., ECE\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EPeter Hesketh, Ph.D., ME\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EWilber A. Lam, MD, Ph.D., BME\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EHang Lu, Ph.D., ChBE\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EThe focus of this work is on a micro-fabrication technique for batch fabrication of a low stiffness fluid force microscope with direct connection to a microfluidic capillary.\u0026nbsp; A fluid force microscope is an AFM probe that has a micro-channeled cantilever, giving it the additional capacity to perform contractile and adhesion measurements, perfusion and cellular manipulation.\u0026nbsp;\u0026nbsp; The abiotic and reversible nature of the device enables decoupling of receptor-ligand associated stimulus responses and enables rapid serial measurements.\u0026nbsp; The major limitations of current fluidic probes are twofold:\u0026nbsp; 1. Current fabrication techniques disenable force microscopy in the pN range, which is physiologically relevant to cell thrust, early adhesion and contraction.\u0026nbsp; 2.\u0026nbsp; The fluidic system interface requires destructive modification of the AFM\u2019s costly mounting assembly.\u0026nbsp; In this work, three enabling design or micro-fabrication techniques are developed and validated: \u003Cbr \/\u003E1. A low stiffness micro-channeled cantilever | The aim is to design and perform both analytical and experimental validation of a thermally decomposable polynorbornene sacrificial resist technique to fabricate low stiffness (\u0026lt;0.1 N\/m) cantilever for high resolution at sub-nN force spectroscopy.\u0026nbsp; Stiffness is first characterized as a function of dimension by a numerical analysis, design and experimental validation follow;\u003Cbr \/\u003E2. A fully integrated microfluidic system | The aim is to design and validate a glass capillary to probe interconnect technique to create a fully integrated fluidic channel avoiding the need for modification of the AFM mounting assembly;\u003Cbr \/\u003E3. A surface to bulk microfluidic channel | The aim is to design and validate a multi-layer and multi-viscosity resist based technique to form a fluidic interface between surface and bulk microfluidic channels for modulation of the pressure at the cantilever terminus.\u003Cbr \/\u003EThe probe is then fabricated and characterized.\u0026nbsp; Biological utility is demonstrated by performing simultaneous measurements showing cell size, adhesion and stiffness on early leukocyte-epithelial cell adhesion and comparing with benchmark technologies.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EA Soft Fluidic Force Microscope Probe for Cellular Force Measurement\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"A Soft Fluidic Force Microscope Probe for Cellular Force Measurement"}],"uid":"27349","created_gmt":"2013-11-18 14:57:00","changed_gmt":"2017-04-13 21:23:49","author":"Floyd Wood","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2013-12-02T10:00:00-05:00","event_time_end":"2013-12-02T13:30:00-05:00","event_time_end_last":"2013-12-02T13:30:00-05:00","gmt_time_start":"2013-12-02 15:00:00","gmt_time_end":"2013-12-02 18:30:00","gmt_time_end_last":"2013-12-02 18:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"6178","name":"BIOE"},{"id":"569","name":"bioengineering"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}