{"274401":{"#nid":"274401","#data":{"type":"event","title":"Petit Institute Seminar","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u0022Exploring T Cell Activation and Differentiation by MS-based Proteomics\u0022\u003Cbr \/\u003E\u003Cbr \/\u003EOreste Acuto, PhD\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EProfessor and Senior Research Fellow\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003ESir William Dunn School of Pathology\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EUniversity of Oxford\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cbr \/\u003ERecent progress in advanced quantitative mass spectrometry allows investigation of cellular proteomes at unprecedented levels of deepness and information abundance, comparable, if not superior to transcriptome analyses.\u003Cbr \/\u003EThese advances permit now the use of limited amounts of biological material, not only to identify predictable missing links and unexpected connection in cellular pathways at the protein and metabolic levels, but also to appreciate changing patterns in cellular functions associated with cell differentiation at systems level.\u003Cbr \/\u003E\u003Cbr \/\u003EDuring the last few years we have widely applied MS-based proteomics to T cell activation and differentiation, a biology and bio-medical research relevant system to understand complex processes set in motion by T cell antigen receptor (TCR) stimulation, in normal and pathological settings.\u003Cbr \/\u003E\u003Cbr \/\u003EI will discuss recent data on the MS-based discovery and functional characterization of Themis, a novel protein implicated in thymus development of conventional T cells. Moreover, I will illustrate various approaches that have allowed reveal global changes protein phosphorylation and\/or protein expression patterns upon TCR stimulation. Finally, I will report unpublished data on extensive cellular \u0022methylome\u0022 studies in T differentiating cells. \u003Cbr \/\u003E\u003Cbr \/\u003EFaculty host - \u003Ca href=\u0022mailto:cheng.zhu@bme.gatech.edu\u0022\u003ECheng Zhu, PhD\u003C\/a\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EThe Petit Institute hosts special guests and visitors throughout the year. \u0026nbsp;These seminars are open to all faculty and students.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Exploring T Cell Activation and Differentiation by MS-based Proteomics\u0022 - Oreste Acuto, PhD - University of Oxford"}],"uid":"27195","created_gmt":"2014-02-06 09:49:31","changed_gmt":"2017-04-13 21:23:13","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2014-04-29T12:00:00-04:00","event_time_end":"2014-04-29T13:00:00-04:00","event_time_end_last":"2014-04-29T13:00:00-04:00","gmt_time_start":"2014-04-29 16:00:00","gmt_time_end":"2014-04-29 17:00:00","gmt_time_end_last":"2014-04-29 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"69773":{"id":"69773","type":"image","title":"Parker H. Petit Institute for Bioengineering and Bioscience","body":null,"created":"1449177264","gmt_created":"2015-12-03 21:14:24","changed":"1475894611","gmt_changed":"2016-10-08 02:43:31","alt":"Parker H. Petit Institute for Bioengineering and Bioscience","file":{"fid":"192836","name":"10c3041-p1-266.jpg","image_path":"\/sites\/default\/files\/images\/10c3041-p1-266_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/10c3041-p1-266_0.jpg","mime":"image\/jpeg","size":2271177,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/10c3041-p1-266_0.jpg?itok=aplnv5pz"}},"274351":{"id":"274351","type":"image","title":"Oreste Acuto, PhD - University of Oxford","body":null,"created":"1449244112","gmt_created":"2015-12-04 15:48:32","changed":"1475894964","gmt_changed":"2016-10-08 02:49:24","alt":"Oreste Acuto, PhD - University of Oxford","file":{"fid":"198721","name":"acuto_0949.jpg","image_path":"\/sites\/default\/files\/images\/acuto_0949_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/acuto_0949_0.jpg","mime":"image\/jpeg","size":66561,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/acuto_0949_0.jpg?itok=9RfVphjv"}}},"media_ids":["69773","274351"],"related_links":[{"url":"http:\/\/www.path.ox.ac.uk\/research\/immunology\/oreste-acuto-group","title":"Acuto lab website"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"},{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"248","name":"IBB"},{"id":"5168","name":"IBB Seminar"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:cheng.zhu@bme.gatech.edu\u0022\u003ECheng Zhu, PhD\u003C\/a\u003E - faculty host\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}