{"295531":{"#nid":"295531","#data":{"type":"event","title":"PhD Defense Presentation - Stephanie Duncanson","body":[{"value":"\u003Cp\u003EAdvisor: Athanassios Sambanis, ChBE\u003Cbr \/\u003E \u003Cbr \/\u003E Committee Members:\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Julia Babensee, BME\u003Cbr \/\u003E \u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Julie Champion, ChBE\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Susan Safley, Emory School of Medicine\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Johnna Temenoff, BME\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EAbstract: \u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EThe development of a bioartificial pancreas (BAP) has the potential to substantially improve the treatment of insulin-dependent diabetes.\u0026nbsp; Composed of insulin-secreting cells encapsulated in a hydrogel material, a BAP may provide superior glycemic regulation compared with conventional exogenous insulin-delivery therapies. Towards this goal, \u03b2- cells or islets encapsulated in alginate microcapsules remain a promising approach. Due to the limited supply of human islets, alternative cell sources are under investigation for incorporation into a BAP, including porcine islets and \u03b2- cell lines. Several challenges remain to clinical implementation, including loss of islet or \u03b2- cell function and viability following transplantation and host response to the transplanted microcapsules.\u003C\/p\u003E\u003Cp\u003EThe objective of this work was to evaluate strategies to improve a BAP by supporting the function and survival of encapsulated islets and \u03b2 -cells.\u0026nbsp; Towards this goal, two areas were explored: 1) the provision of pro-survival and insulinotropic factors, namely, CXCL12 and GLP-1, to encapsulated islets and \u03b2-cells and 2) modification of the alginate microcapsule to confer long-term resistance to host cell adhesion.\u0026nbsp;\u0026nbsp;\u003C\/p\u003E\u003Cp\u003ETo achieve the first objective, methods to deliver both pro-survival and insulinotropic factors to a BAP were developed and their effects on encapsulated \u03b2-cells and islets were studied, both \u003Cem\u003Ein vitro\u003C\/em\u003E and \u003Cem\u003Ein vivo\u003C\/em\u003E. Results demonstrate that delivery of both pro-survival and insulinotropic factors are a promising strategy to prolong the survival and function of a BAP.\u0026nbsp; To reduce host cell adhesion to the microcapsule, we employed covalent conjugation of PEG to the surface of alginate-PLL capsules to replace the un-crosslinked layer of alginate used in traditional alginate-PLL-alginate (APA) microcapsules.\u0026nbsp; Results demonstrate that while PEGylation of alginate-PLL microcapsules initially reduced host cell adhesion over 2 weeks \u003Cem\u003Ein vivo \u003C\/em\u003Ecompared with APA capsules, the PEG coating did not provide long-term protection over 3 months.\u0026nbsp; Taken together, these studies represent a multipronged approach towards improving the duration of BAP function, with the ultimate goal of advancing this technology to the clinic.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EPhD Defense Presentation-\u0026nbsp;\u0022Improving the bioartificial pancreas: Investigation of the effects of pro-survival and insulinotropic factor delivery and development of PEGylated alginate microcapsules to support the function and survival of encapsulated islets and beta cells\u0022- Stephanie Duncanson\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Improving the bioartificial pancreas: Investigation of the effects of pro-survival and insulinotropic factor delivery and development of PEGylated alginate microcapsules to support the function and survival of encapsulated islets and beta cells\u0022"}],"uid":"27917","created_gmt":"2014-05-07 09:57:59","changed_gmt":"2017-04-13 21:22:37","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2014-05-19T19:00:00-04:00","event_time_end":"2014-05-19T21:00:00-04:00","event_time_end_last":"2014-05-19T21:00:00-04:00","gmt_time_start":"2014-05-19 23:00:00","gmt_time_end":"2014-05-20 01:00:00","gmt_time_end_last":"2014-05-20 01:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/sambanis.chbe.gatech.edu\/","title":"http:\/\/sambanis.chbe.gatech.edu\/"}],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}