{"403091":{"#nid":"403091","#data":{"type":"event","title":"Ph.D Proposal by Gregory Holst","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EGregory Holst\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EPhD Thesis Proposal\u003C\/p\u003E\u003Cp\u003ETime: Thursday, May 21, 2015 , 1-3pm\u003C\/p\u003E\u003Cp\u003ELocation: \u0026nbsp;4211 MRDC\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EAdvisor\u003C\/strong\u003E: Craig R. Forest, PhD (Georgia Institute of Technology, Mechanical Engineering)\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECommittee Members\u003C\/strong\u003E:\u003C\/p\u003E\u003Cp\u003EGarrett B.\u0026nbsp;Stanley, PhD (Georgia Institute of Technology, Biomedical Engineering)\u003C\/p\u003E\u003Cp\u003ETodd Sulchek, PhD (Georgia Institute of Technology, Mechanical Engineering)\u003C\/p\u003E\u003Cp\u003EHongkui Zeng, PhD (Allen Institute for Brain Science)\u003C\/p\u003E\u003Cp\u003EEdward S. Boyden, PhD \u0026nbsp;(Massachusetts Institute of Technology, Biological Engineering and Brain and Cognitive Sciences)\u003C\/p\u003E\u003Cp\u003ESuhasa B. Kodandaramaiah, PhD (University of Minnesota, Mechanical Engineering)\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ETitle\u003C\/strong\u003E: In vivo serial patch clamp robotics for cell type identification in the mouse visual cortex\u003C\/p\u003E\u003Cp\u003E\u003Cbr \/\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cbr \/\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cbr \/\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EPatch-clamping, the gold standard technique for measuring trans-membrane voltages and currents in neurons, involves delicately resting a 1 \u03bcm diameter pipette against a cell to create an intimate electrical and mechanical connection between the pipette tip and the cell membrane. From there, it is possible to record essentially interference-free single-neuron \u201cspikes\u201d in membrane voltage. These spikes are the primary method of inter-neuronal communication in the nervous system.\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EThe experimental procedure to obtain these high-fidelity recordings is considered an art form performed in vivo by a small number of highly trained individuals.\u0026nbsp; Previous work has introduced mechanical and electrical automation techniques, or \u0022autopatching,\u0022 to reduce the cognitive load and the required training to obtain these recordings, but the low yield is still a major limitation and requires many attempts to obtain a single recording. \u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EThis work introduces additional robotic tools to completely automate serial patch clamp recording attempts.\u0026nbsp; Electrical and mechanical hardware and software algorithms have been developed to automate pipette manipulation, specifically, pneumatic control, electrical control, precise positioning, replacement, filling, wire threading, and storage.\u0026nbsp; Taken together, these tools enable the first completely autonomous, serial patch clamp recording attempts in the living brain.\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EThese robotic tools also enable more difficult experiments that combine patch clamp recording with other techniques such as biocytin filling for morphological reconstruction.\u0026nbsp; Progress towards a survey of 50 cells in the visual cortex will be presented to establish cell type identification schemes based on coupled electrophysiology and morphology.\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E \u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"In vivo serial patch clamp robotics for cell type identification in the mouse visual cortex"}],"uid":"27707","created_gmt":"2015-05-08 12:05:03","changed_gmt":"2016-10-08 02:11:57","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2015-05-21T14:00:00-04:00","event_time_end":"2015-05-21T16:00:00-04:00","event_time_end_last":"2015-05-21T16:00:00-04:00","gmt_time_start":"2015-05-21 18:00:00","gmt_time_end":"2015-05-21 20:00:00","gmt_time_end_last":"2015-05-21 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"1808","name":"graduate students"},{"id":"913","name":"PhD"},{"id":"3395","name":"proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}