{"467751":{"#nid":"467751","#data":{"type":"event","title":"Breakfast Club Seminar","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u0022Visualizing Biological Structures by Cryo-EM: From Proteins to Cells\u0022\u0026nbsp;\u003Cbr \/\u003E\u003Cbr \/\u003EIngeborg Schmidt-Krey, Ph.D.\u003Cbr \/\u003EAssociate Professor\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003ESchool of Chemistry\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EGeorgia Tech\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\u003Cp\u003EEukaryotic membrane proteins comprise approximately 60% of all drug targets and are consequently immensely important for biomedical research. Despite their importance, only few could thus far be studied at the structural level. My research focuses on the crystallization, structure and function of eukaryotic membrane proteins.\u003C\/p\u003E\u003Cp\u003EElectron crystallography is the main tool employed to study these proteins in my laboratory. The approach of 2D crystallization and electron crystallography is particularly suitable for highly fragile membrane proteins such as many eukaryotic ones. Initially, this involves testing of conditions for growing two-dimensional (2D) crystals, usually by reconstituting the detergent-solubilized membrane protein into a phospholipid bilayer. Once crystallization parameters have been identified by electron microscopy of negatively stained samples, electron cryo-microscopy (cryo-EM) is employed to collect high-resolution data. The structure is then obtained by image processing.\u003C\/p\u003E\u003Cp\u003EAlthough electron crystallographic methods are well developed, little is known about the factors important in 2D crystallization, and screening protocols as for 3D crystallization do not exist. An important sideline of my research interests aims at developing screening methods and strategies for 2D crystallization and understanding the underlying mechanisms. Further directions of methods development are geared towards cryo-EM data collection and analysis.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cbr \/\u003E\u003Cem\u003EThe Parker H. Petit Institute for Bioengineering and Bioscience, an internationally recognized hub of multidisciplinary research at the Georgia Institute of Technology, brings engineers, scientists, and clinicians together to solve some of the world\u2019s most complex health challenges. With 18 research centers, more than 180 faculty members, and $24 million in state-of-the-art facilities, the Petit Institute is translating scientific discoveries into game-changing solutions to solve real-world problems.\u003C\/em\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EThe Petit Institute Breakfast Club seminar series was started with the spirit of the Institute\u0027s interdisciplinary mission in mind and started to feature local Petit Institute faculty member\u0027s research in a seminar format. Faculty are often asked to speak at other universities and conferences, but rarely present at their home institution, this seminar series is an attempt to close that gap. The Petit Institute Breakfast Club is open to anyone in the bio-community.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Visualizing Biological Structures by Cryo-EM: From Proteins to Cells\u0022 - Ingeborg Schmidt-Krey, Ph.D. - Georgia Tech"}],"uid":"27195","created_gmt":"2015-11-09 15:54:43","changed_gmt":"2017-04-13 21:17:41","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2016-04-19T13:30:00-04:00","event_time_end":"2016-04-19T14:30:00-04:00","event_time_end_last":"2016-04-19T14:30:00-04:00","gmt_time_start":"2016-04-19 17:30:00","gmt_time_end":"2016-04-19 18:30:00","gmt_time_end_last":"2016-04-19 18:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"70917":{"id":"70917","type":"image","title":"Breakfast Club Seminar Series","body":null,"created":"1449177328","gmt_created":"2015-12-03 21:15:28","changed":"1475894625","gmt_changed":"2016-10-08 02:43:45","alt":"Breakfast Club Seminar Series","file":{"fid":"193471","name":"logobldg_graphic.png","image_path":"\/sites\/default\/files\/images\/logobldg_graphic_0.png","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/logobldg_graphic_0.png","mime":"image\/png","size":241588,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/logobldg_graphic_0.png?itok=x84xf3Mz"}}},"media_ids":["70917"],"related_links":[{"url":"https:\/\/www.chemistry.gatech.edu\/people\/schmidt-krey\/ingeborg","title":"Schmidt-Krey profile"},{"url":"http:\/\/petitinstitute.gatech.edu\/breakfast-club","title":"Breakfast Club Seminar schedule"}],"groups":[{"id":"1254","name":"Wallace H. Coulter Dept. of Biomedical Engineering"},{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"1808","name":"graduate students"},{"id":"248","name":"IBB"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:colly.mitchell@ibb.gatech.edu\u0022\u003EColly Mitchell\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}