{"497281":{"#nid":"497281","#data":{"type":"news","title":"One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil\u2013water interfaces","body":[{"value":"\u003Cp\u003ECongratulations to \u003Ca href=\u0022http:\/\/www.physics.gatech.edu\/user\/peter-yunker\u0022\u003EProf. Peter Yunker\u003C\/a\u003E on his newly published paper in PNAS titled, \u201cOne-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces\u0022.\u003C\/p\u003E\u003Ch3\u003ESummary\u003C\/h3\u003E\u003Cp\u003ESingle-span membrane proteins (ssMPs) are anchored by single hydrophobic helices to cell surfaces, where they mediate cell\u2013cell communications. Unfortunately, hydrophobic helices also cause aggregation in solution, rendering ssMPs nonfunctional. We discovered that in vitro-synthesized ssMPs localize on an oil drop\u2019s surface, preventing aggregation of ssMPs in solution and promoting assembly of functional structures on the drop\u2019s surface. We use this approach to synthesize and display apoptosis-inducing ssMPs and show that these \u201cdeath drops\u201d are functional and can kill cultured cancer cells. Our results illustrate a one-pot method for rapid synthesis and assembly of functional ssMPs, which is facilitated by the hydrophobic interaction rather than limited by it. Such functionalized oil drops represent a platform to communicate with cells.\u003C\/p\u003E\u003Cp\u003ERead more: \u003Ca href=\u0022http:\/\/www.pnas.org\/content\/113\/3\/608.full\u0022\u003Ehttp:\/\/www.pnas.org\/content\/113\/3\/608.full\u003C\/a\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003ECongratulations to Prof. Peter Yunker on his newly published paper in PNAS titled, \u201cOne-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces\u0022.\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Prof. Peter Yunker\u0027s newly published paper in PNAS."}],"uid":"28004","created_gmt":"2016-02-09 12:37:22","changed_gmt":"2016-10-08 03:20:38","author":"Dione Morton","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2016-02-09T00:00:00-05:00","iso_date":"2016-02-09T00:00:00-05:00","tz":"America\/New_York"},"extras":[],"hg_media":{"497191":{"id":"497191","type":"image","title":"Figure 1","body":null,"created":"1455120000","gmt_created":"2016-02-10 16:00:00","changed":"1475895256","gmt_changed":"2016-10-08 02:54:16","alt":"Figure 1","file":{"fid":"204622","name":"yunkerpnas.jpg","image_path":"\/sites\/default\/files\/images\/yunkerpnas_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/yunkerpnas_0.jpg","mime":"image\/jpeg","size":275558,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/yunkerpnas_0.jpg?itok=u8XzgCv7"}}},"media_ids":["497191"],"groups":[{"id":"126011","name":"School of Physics"}],"categories":[{"id":"150","name":"Physics and Physical Sciences"}],"keywords":[{"id":"5230","name":"Biophysics"},{"id":"171656","name":"cell-free protein synthesis"},{"id":"7305","name":"hydrophobic"},{"id":"171657","name":"membrane protein"},{"id":"166937","name":"School of Physics"},{"id":"171658","name":"self assembly"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003ESchool of Physics\u003Cbr \/\u003E837 State Street\u003Cbr \/\u003EAtlanta, Georgia 30332-0430\u003C\/p\u003E","format":"limited_html"}],"email":["dione.morton@physics.gatech.edu"],"slides":[],"orientation":[],"userdata":""}}}