{"594464":{"#nid":"594464","#data":{"type":"event","title":"BioE PhD Defense Announcement- Faisal Ahmed","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003ECommittee members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Cyrus Aidun (Advisor)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Gilda Barabino(Advisor)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Edward Botchwey\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Brandon Dixon\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Wilbur Lam\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle:\u0026nbsp;MICROFLUIDIC DEVICES FOR STIFFNESS DEPENDENT ENRICHMENT OF\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ERED BLOOD CELL SUBPOPULATION\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERed blood cells being the most dominant cell type of blood are often the target of many\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ehematologic diseases such as sickle cell disease, malaria, spherocytosis and some types of\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ecancers. In addition to affecting biological properties, these diseases also alter biomechanical\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eproperties such as morphology, size and stiffness of red blood cells. Separating or enriching\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethe cellular components of blood into subpopulation based on their bio-mechanical\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eproperties and analyzing them have the potential to lead to enhanced strategies for assessment\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eand treatment of these diseases. Current techniques and equipment for diseased cell\u003C\/p\u003E\r\n\r\n\u003Cp\u003Esample enrichment are time consuming, expensive and need well trained professionals to\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ebe conducted. Microfluidic platform based red blood cell enrichment device is one of the\u003C\/p\u003E\r\n\r\n\u003Cp\u003Emost promising technologies that are currently the subject of considerable interest among\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eresearchers because of its low cost, high throughput, easy operation and the potential to\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edo enrichment within the physiological flow condition. In this research work, microfluidic\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edevices were designed, fabricated and tested for enriching red blood cell subpopulations\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ebased on their stiffness from a mixture of stiff and normal red blood cells. In the first portion\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eof the work, lab developed numerical simulation tools were deployed to study stiffness\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edependent margination pattern of red blood cells in high aspect ratio straight microchannels\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ewith rectangular cross-section. Stiff red blood cells were observed to marginate near the\u003C\/p\u003E\r\n\r\n\u003Cp\u003Echannel walls whereas normal (and hence more deformable) red blood cells were observed\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eto marginate around the center line of the channel regardless whether cell-cell interaction\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ewas significant or not. Cells of different stiffness reached to their equilibrium locations\u003C\/p\u003E\r\n\r\n\u003Cp\u003Efaster in channels with smaller cross sections. Increasing flow Reynolds number and hence\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethe flow rate resulted in stronger segregation between normal and stiff red blood cells for\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethe whole range of Reynolds numbers for which simulations were run. Increasing cell volume\u003C\/p\u003E\r\n\r\n\u003Cp\u003Efraction in solution also boosted separation between cells of different stiffness. Based\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eon the findings of the simulations, two types of cell enrichment devices were designed and\u003C\/p\u003E\r\n\r\n\u003Cp\u003Efabricated, simple straight channel device and multistep device. The simple straight channel\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edevice was tested for a wide range of flow Reynolds number and cell volume fractions.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESimple straight channels were observed to perform better with increasing flow Reynolds\u003C\/p\u003E\r\n\r\n\u003Cp\u003Enumber and cell volume fraction up to certain threshold for each of them, and after that\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethreshold there was no significant improvement of performance. Numerical simulations\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ewere conducted with parameters matching with some of the experiments and the results\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eobtained were remarkably close to those from the experiments. Statistical analysis on experimental\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edata found the effect of individual parameters, flow Reynolds number and cell\u003C\/p\u003E\r\n\r\n\u003Cp\u003Evolume fraction, to be significant. It also revealed that there was significant interaction between\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethe factors flow Reynolds number and volume fraction. This implies that the extent\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eof the effect of one factor (e.g. flow Reynolds number) changes when the value of the\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eother factor (e.g. volume fraction) varies. The multistep device was also tested for different\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ecombinations of flow Reynolds number and cell volume fraction and, was observed\u003C\/p\u003E\r\n\r\n\u003Cp\u003Eto perform 1.6 times to 3.15 times better in enriching stiff cells from a mixture of stiff and\u003C\/p\u003E\r\n\r\n\u003Cp\u003Enormally deformable red blood cells. To our knowledge this is the first study that incorporated\u003C\/p\u003E\r\n\r\n\u003Cp\u003Esuch rigorous multiphysics simulations to support experimental study on stiffness\u003C\/p\u003E\r\n\r\n\u003Cp\u003Edependent margination of red blood cells in straight micro-channels. This research work\u003C\/p\u003E\r\n\r\n\u003Cp\u003Erevealed previously unreported information about stiffness dependent cell enrichment with\u003C\/p\u003E\r\n\r\n\u003Cp\u003Esimple straight channel microfluidic device and proposed a new device that performed significantly\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ebetter than the simple straight channel device.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Defense Presentation-\u0026nbsp;\u0026quot;MICROFLUIDIC DEVICES FOR STIFFNESS DEPENDENT ENRICHMENT OF RED BLOOD CELL SUBPOPULATION\u0026quot;- Faisal Ahmed\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022MICROFLUIDIC DEVICES FOR STIFFNESS DEPENDENT ENRICHMENT OF RED BLOOD CELL SUBPOPULATION\u0022"}],"uid":"27917","created_gmt":"2017-08-15 20:02:48","changed_gmt":"2017-08-15 20:02:48","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2017-08-29T10:00:00-04:00","event_time_end":"2017-08-29T12:00:00-04:00","event_time_end_last":"2017-08-29T12:00:00-04:00","gmt_time_start":"2017-08-29 14:00:00","gmt_time_end":"2017-08-29 16:00:00","gmt_time_end_last":"2017-08-29 16:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003ELaura Paige\u003C\/p\u003E\r\n\r\n\u003Cp\u003E404-385-6655\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}