{"595293":{"#nid":"595293","#data":{"type":"event","title":"Assessing Metabolism and Interspecies Interactions in Microbial Symbioses and Microbial Communities via Metaproteomics","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EManuel Kleiner\u003Cbr \/\u003E\r\nAssistant Professor\u003Cbr \/\u003E\r\nDepartment of Plant and Microbial Biology \u0026amp; \u003Ca href=\u0022https:\/\/facultyclusters.ncsu.edu\/clusters\/microbiomes-and-complex-microbial-communities\/\u0022\u003EMicrobiomes and Complex Microbial Communities Cluster\u003C\/a\u003E\u003Cbr \/\u003E\r\nNorth Carolina State University\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract\u003C\/strong\u003E\u003Cbr \/\u003E\r\nMetaproteomics refers to all high-throughput methods used to identify and quantify proteins in microbial communities to determine the metabolic pathways and physiological functions that are relevant under a given environmental condition.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EInitially, metaproteomics was a makeshift adaptation of proteomic methods used for single (pure-culture) organisms, but not for complex communities of uncultured organisms. Only recently have some research groups begun to develop dedicated metaproteomic approaches that provide new insights into microbial-community functioning.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EManuel Kleiner will give an overview of the\u0026nbsp;state of the art in metaproteomics and use several examples of exciting discoveries to illustrate what kind of questions can be addressed with metapoteomics.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EKleiner will present two metaproteomic approaches developed only\u0026nbsp;recently in his lab. The first provides a method to assess\u0026nbsp;species biomass contributions in microbial communities. The second allows us to determine the stable carbon isotope composition of individual species in microbial communities, which can provide clues about the\u0026nbsp;metabolic pathways and food sources used by an organism.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ETo develop these approaches into robust and reproducible tools, Kleiner used mock microbial communities for method development, testing, and validation. He will share lessons from using these mock communities.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Ca href=\u0022https:\/\/pmb.cals.ncsu.edu\/people\/people-table\/dr-manuel-kleiner\/\u0022\u003EMore about Manuel Kleiner\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHost: Frank Stewart\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EManuel Kleiner will give an overview of the\u0026nbsp;state of the art in metaproteomics and use several examples of exciting discoveries to illustrate what kind of questions can be addressed with metapoteomics.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"A School of Biological Sciences seminar by Manuel Kleiner, North Carolina State University"}],"uid":"27964","created_gmt":"2017-08-30 14:59:54","changed_gmt":"2017-09-19 21:14:18","author":"Jasmine Martin","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2017-10-19T11:55:00-04:00","event_time_end":"2017-10-19T12:55:00-04:00","event_time_end_last":"2017-10-19T12:55:00-04:00","gmt_time_start":"2017-10-19 15:55:00","gmt_time_end":"2017-10-19 16:55:00","gmt_time_end_last":"2017-10-19 16:55:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"596209":{"id":"596209","type":"image","title":"Manuel Kleiner, North Carolina State University","body":null,"created":"1505854328","gmt_created":"2017-09-19 20:52:08","changed":"1505854328","gmt_changed":"2017-09-19 20:52:08","alt":"","file":{"fid":"227213","name":"Manuel_Kleiner-e1502929978489.jpg","image_path":"\/sites\/default\/files\/images\/Manuel_Kleiner-e1502929978489.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/Manuel_Kleiner-e1502929978489.jpg","mime":"image\/jpeg","size":29119,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/Manuel_Kleiner-e1502929978489.jpg?itok=TSIVsyCx"}}},"media_ids":["596209"],"groups":[{"id":"1278","name":"College of Sciences"},{"id":"1275","name":"School of Biological Sciences"},{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[{"id":"25111","name":"Frank Stewart"},{"id":"166892","name":"School of Biological Sciences Seminar"},{"id":"175354","name":"Manuel Kleiner"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:frank.stewart@biology.gatech.edu\u0022\u003EFrank Stewart\u003C\/a\u003E\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}