{"601215":{"#nid":"601215","#data":{"type":"event","title":"BME Seminar Speaker - Yarui Diao, Ph.D.*","body":[{"value":"\u003Cp\u003E*** BME Faculty Candidate\u0026nbsp;***\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Ch3\u003E\u003Cstrong\u003EYarui Diao, Ph.D.*\u003C\/strong\u003E\u003Cbr \/\u003E\r\nPostdoctoral Fellow\u003Cbr \/\u003E\r\nLudwig Institute for Cancer Research\u003Cbr \/\u003E\r\nSan Diego\u003C\/h3\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Ch2\u003EChopping Away at \u0026quot;Junk\u0026quot; DNA: Using High-throughput CRISPR\/Cas9 to Uncover the Role of Non-coding Genome Sequence\u003C\/h2\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EABSTRACT\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe precise transcriptional regulation involves the interplay between cis- (e.g. promoters and enhancers) and trans- (e.g. transcription factors) regulatory factors. In the human genome, millions of cis-regulatory elements have been predicted through the analysis of chromatin biochemical features. Most strikingly, these regulatory sequence harbors \u0026gt;90% of human diseases associated genetic variants, leading to the theory that genetic lesions in the regulatory elements can substantially contribute to human diseases. However, for most of the cis-regulatory elements, their functional consequence in the native chromatin context remains largely unknown. It is also challenging to annotate the enhancer regulatory target gene. To fill such knowledge gap, I developed high-throughput CRISPR\/Cas9 screen strategies that allow for genome-wide functional characterization of non-coding regulatory sequence in the native chromatin context. My work demonstrated that many gene promoters act as long-range enhancers, and challenged the traditional concept that promoter and enhancer are distinct elements. I also mapped the promoter-centered long-range chromatin interactions in 27 human tissue types, to interpret the regulatory target gene of enhancer and disease associated genetic variants. In the future, I will integrate the cutting-edge functional genomics tools with muscle stem cell model and iPSC technology to improve the understanding of the mechanism that control muscle stem cell regenerative potency, and in the long-run, to develop stem cell based therapies to treat muscular disorders which affect aging, cancer, and a broad spectrum of human diseases.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHost: James Dahlman, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"BME Seminar Speaker - Yarui Diao, Ph.D.*"}],"uid":"27513","created_gmt":"2018-01-23 18:56:59","changed_gmt":"2018-02-01 14:31:52","author":"Walter Rich","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-02-01T10:30:00-05:00","event_time_end":"2018-02-01T11:30:00-05:00","event_time_end_last":"2018-02-01T11:30:00-05:00","gmt_time_start":"2018-02-01 15:30:00","gmt_time_end":"2018-02-01 16:30:00","gmt_time_end_last":"2018-02-01 16:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"601300":{"id":"601300","type":"image","title":"Yarui Diao, PhD*","body":null,"created":"1516822388","gmt_created":"2018-01-24 19:33:08","changed":"1516822388","gmt_changed":"2018-01-24 19:33:08","alt":"Yarui Diao, PhD*","file":{"fid":"229162","name":"Yarui Diao screenshot_233.png","image_path":"\/sites\/default\/files\/images\/Yarui%20Diao%20screenshot_233.png","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/Yarui%20Diao%20screenshot_233.png","mime":"image\/png","size":386123,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/Yarui%20Diao%20screenshot_233.png?itok=Gs_0-VO8"}}},"media_ids":["601300"],"groups":[{"id":"1254","name":"Wallace H. Coulter Dept. of Biomedical Engineering"}],"categories":[],"keywords":[{"id":"1612","name":"BME"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EJames Dahlman\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}