{"61055":{"#nid":"61055","#data":{"type":"news","title":"New Biosensing Technology Could Facilitate Personalized Medicine","body":[{"value":"\u003Cp\u003EThe multi-welled microplate, long a standard tool in biomedical research and diagnostic laboratories, could become a thing of the past thanks to new electronic biosensing technology developed by a team of microelectronics engineers and biomedical scientists at the Georgia Institute of Technology.\u003C\/p\u003E\u003Cp\u003EEssentially arrays of tiny test tubes, microplates have been used for decades to simultaneously test multiple samples for their responses to chemicals, living organisms or antibodies. Fluorescence or color changes in labels associated with compounds on the plates can signal the presence of particular proteins or gene sequences. \u003C\/p\u003E\u003Cp\u003EThe researchers hope to replace these microplates with modern microelectronics technology, including disposable arrays containing thousands of electronic sensors connected to powerful signal processing circuitry. If they\u0027re successful, this new electronic biosensing platform could help realize the dream of personalized medicine by making possible real-time disease diagnosis -- potentially in a physician\u2019s office -- and by helping select individualized therapeutic approaches. \u003C\/p\u003E\u003Cp\u003E\u0022This technology could help facilitate a new era of personalized medicine,\u0022 said John McDonald, chief research scientist at the Ovarian Cancer Institute in Atlanta and a professor in the Georgia Tech School of Biology. \u0022A device like this could quickly detect in individuals the gene mutations that are indicative of cancer and then determine what would be the optimal treatment. There are a lot of potential applications for this that cannot be done with current analytical and diagnostic technology.\u0022 \u003C\/p\u003E\u003Cp\u003EFundamental to the new biosensing system is the ability to electronically detect markers that differentiate between healthy and diseased cells. These markers could be differences in proteins, mutations in DNA or even specific levels of ions that exist at different amounts in cancer cells. Researchers are finding more and more differences like these that could be exploited to create fast and inexpensive electronic detection techniques that don\u0027t rely on conventional labels. \u003C\/p\u003E\u003Cp\u003E\u0022We have put together several novel pieces of nanoelectronics technology to create a method for doing things in a very different way than what we have been doing,\u0022 said Muhannad Bakir, an associate professor in Georgia Tech\u0027s School of Electrical and Computer Engineering. \u0022What we are creating is a new general-purpose sensing platform that takes advantage of the best of nanoelectronics and three-dimensional electronic system integration to modernize and add new applications to the old microplate application. This is a marriage of electronics and molecular biology.\u0022 \u003C\/p\u003E\u003Cp\u003EThe three-dimensional sensor arrays are fabricated using conventional low-cost, top-down microelectronics technology. Though existing sample preparation and loading systems may have to be modified, the new biosensor arrays should be compatible with existing work flows in research and diagnostic labs. \u003C\/p\u003E\u003Cp\u003E\u201cWe want to make these devices simple to manufacture by taking advantage of all the advances made in microelectronics, while at the same time not significantly changing usability for the clinician or researcher,\u201d said Ramasamy Ravindran, a graduate research assistant in Georgia Tech\u2019s Nanotechnology Research Center and the School of Electrical and Computer Engineering. \u003C\/p\u003E\u003Cp\u003EA key advantage of the platform is that sensing will be done using low-cost, disposable components, while information processing will be done by reusable conventional integrated circuits connected temporarily to the array. Ultra-high density spring-like mechanically compliant connectors and advanced \u0022through-silicon vias\u0022 will make the electrical connections while allowing technicians to replace the biosensor arrays without damaging the underlying circuitry. \u003C\/p\u003E\u003Cp\u003ESeparating the sensing and processing portions allows fabrication to be optimized for each type of device, notes Hyung Suk Yang, a graduate research assistant also working in the Nanotechnology Research Center. Without the separation, the types of materials and processes that can be used to fabricate the sensors are severely limited. \u003C\/p\u003E\u003Cp\u003EThe sensitivity of the tiny electronic sensors can often be greater than current systems, potentially allowing diseases to be detected earlier. Because the sample wells will be substantially smaller than those of current microplates -- allowing a smaller form factor -- they could permit more testing to be done with a given sample volume. \u003C\/p\u003E\u003Cp\u003EThe technology could also facilitate use of ligand-based sensing that recognizes specific genetic sequences in DNA or messenger RNA. \u0022This would very quickly give us an indication of the proteins that are being expressed by that patient, which gives us knowledge of the disease state at the point-of-care,\u0022 explained Ken Scarberry, a postdoctoral fellow in McDonald\u0027s lab. \u003C\/p\u003E\u003Cp\u003ESo far, the researchers have demonstrated a biosensing system with silicon nanowire sensors in a 16-well device built on a one-centimeter by one-centimeter chip. The nanowires, just 50 by 70 nanometers, differentiated between ovarian cancer cells and healthy ovarian epithelial cells at a variety of cell densities. \u003C\/p\u003E\u003Cp\u003ESilicon nanowire sensor technology can be used to simultaneously detect large numbers of different cells and biomaterials without labels. Beyond that versatile technology, the biosensing platform could accommodate a broad range of other sensors \u2013 including technologies that may not exist yet. Ultimately, hundreds of thousands of different sensors could be included on each chip, enough to rapidly detect markers for a broad range of diseases. \u003C\/p\u003E\u003Cp\u003E\u0022Our platform idea is really sensor agnostic,\u0022 said Ravindran. \u0022It could be used with a lot of different sensors that people are developing. It would give us an opportunity to bring together a lot of different kinds of sensors in a single chip.\u0022 \u003C\/p\u003E\u003Cp\u003EGenetic mutations can lead to a large number of different disease states that can affect a patient\u0027s response to disease or medication, but current labeled sensing methods are limited in their ability to detect large numbers of different markers simultaneously. \u003C\/p\u003E\u003Cp\u003EMapping single nucleotide polymorphisms (SNPs), variations that account for approximately 90 percent of human genetic variation, could be used to determine a patient\u0027s propensity for a disease, or their likelihood of benefitting from a particular intervention. The new biosensing technology could enable caregivers to produce and analyze SNP maps at the point-of-care. \u003C\/p\u003E\u003Cp\u003EThough many technical challenges remain, the ability to screen for thousands of disease markers in real-time has biomedical scientists like McDonald excited. \u003C\/p\u003E\u003Cp\u003E\u0022With enough sensors in there, you could theoretically put all possible combinations on the array,\u0022 he said. \u0022This has not been considered possible until now because making an array large enough to detect them all with current technology is probably not feasible. But with microelectronics technology, you can easily include all the possible combinations, and that changes things.\u0022 \u003C\/p\u003E\u003Cp\u003EPapers describing the biosensing device were presented at the Electronic Components and Technology Conference and the International Interconnect Technology conference in June 2010. The research has been supported in part by the National Nanotechnology Infrastructure Network (NNIN), Georgia Tech\u0027s Integrative BioSystems Institute (IBSI) and the Semiconductor Research Corporation. \u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EResearch News \u0026amp; Publications Office\u003Cbr \/\u003EGeorgia Institute of Technology\u003Cbr \/\u003E75 Fifth Street, N.W., Suite 314\u003Cbr \/\u003EAtlanta, Georgia 30308 USA\u003C\/strong\u003E \u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EMedia Relations Contacts\u003C\/strong\u003E: John Toon (404-894-6986)(\u003Ca href=\u0022mailto:jtoon@gatech.edu\u0022\u003Ejtoon@gatech.edu\u003C\/a\u003E) or Abby Vogel Robinson (404-385-3364)(\u003Ca href=\u0022mailto:abby@innovate.gatech.edu\u0022\u003Eabby@innovate.gatech.edu\u003C\/a\u003E). \u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EWriter\u003C\/strong\u003E: John Toon \u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EThe microplate, a standard tool in biomedical research and diagnostic laboratories, could become a thing of the past thanks to new electronic biosensing technology developed by a team of microelectronics engineers and biomedical scientists.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"A new electronic microplate offers new biosensing options."}],"uid":"27303","created_gmt":"2010-09-20 00:00:00","changed_gmt":"2016-10-08 03:07:27","author":"John Toon","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2010-09-20T00:00:00-04:00","iso_date":"2010-09-20T00:00:00-04:00","tz":"America\/New_York"},"extras":[],"hg_media":{"61056":{"id":"61056","type":"image","title":"Comparing old and new microplates","body":null,"created":"1449176308","gmt_created":"2015-12-03 20:58:28","changed":"1475894531","gmt_changed":"2016-10-08 02:42:11","alt":"Comparing old and new microplates","file":{"fid":"191268","name":"teb94763.jpg","image_path":"\/sites\/default\/files\/images\/teb94763_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/teb94763_0.jpg","mime":"image\/jpeg","size":1422856,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/teb94763_0.jpg?itok=d-o8ode1"}},"61057":{"id":"61057","type":"image","title":"Comparing old and new microplates","body":null,"created":"1449176308","gmt_created":"2015-12-03 20:58:28","changed":"1475894531","gmt_changed":"2016-10-08 02:42:11","alt":"Comparing old and new microplates","file":{"fid":"191269","name":"tqo94763.jpg","image_path":"\/sites\/default\/files\/images\/tqo94763_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/tqo94763_0.jpg","mime":"image\/jpeg","size":867486,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/tqo94763_0.jpg?itok=TqqCosQx"}},"61058":{"id":"61058","type":"image","title":"Binding ligands to silicon nanowires","body":null,"created":"1449176308","gmt_created":"2015-12-03 20:58:28","changed":"1475894531","gmt_changed":"2016-10-08 02:42:11","alt":"Binding ligands to silicon nanowires","file":{"fid":"191270","name":"tmm94763.jpg","image_path":"\/sites\/default\/files\/images\/tmm94763_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/tmm94763_0.jpg","mime":"image\/jpeg","size":1082245,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/tmm94763_0.jpg?itok=J1La386t"}}},"media_ids":["61056","61057","61058"],"related_links":[{"url":"http:\/\/www.ece.gatech.edu\/","title":"School of Electrical and Computer Engineering"},{"url":"http:\/\/www.biology.gatech.edu\/","title":"School of Biology"},{"url":"http:\/\/www.mirc.gatech.edu\/","title":"Microelectronics Research Center"},{"url":"http:\/\/ovariancancerinstitute.org\/","title":"Ovarian Cancer Institute"}],"groups":[{"id":"1188","name":"Research Horizons"}],"categories":[{"id":"140","name":"Cancer Research"},{"id":"145","name":"Engineering"},{"id":"146","name":"Life Sciences and Biology"},{"id":"149","name":"Nanotechnology and Nanoscience"},{"id":"135","name":"Research"}],"keywords":[{"id":"10677","name":"biosensing"},{"id":"10678","name":"diagnostic"},{"id":"10676","name":"microplate"},{"id":"10679","name":"personalized medicine"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Cstrong\u003EJohn Toon\u003C\/strong\u003E\u003Cbr \/\u003EResearch News \u0026amp; Publications Office\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=jt7\u0022\u003EContact John Toon\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-6986\u003C\/strong\u003E\u003C\/p\u003E","format":"limited_html"}],"email":["jtoon@gatech.edu"],"slides":[],"orientation":[],"userdata":""}}}