{"644611":{"#nid":"644611","#data":{"type":"event","title":"QBioS Student Seminar Series - Mengshi Zhang","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u003Cem\u003EQuantification of mRNA and protein and integration with their half-life dynamics in Aggregatibacter actinomycetemcomitans\u003C\/em\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBacterial gene expression and regulation mainly involves coupled processes of transcription, translation and degradation of messenger RNAs (mRNAs) and proteins. Limited efforts have been made to quantify the combined effects of these gene regulatory events globally. Here we aim to understand genome-wide gene expression control and to evaluate if RNA level is a good proxy to predict protein abundance in model organisms. I first analyzed the correlation of mRNA and protein abundance in \u003Cem\u003EPseudomonas aeruginosa (PA).\u003C\/em\u003E Our \u003Cem\u003EPA\u003C\/em\u003E data suggest that mRNA abundance alone cannot precisely predict protein abundance (~60%). I also measured mRNA and protein expression profiles in \u003Cem\u003EAggregatibacter actinomycetemcomitans (Aa)\u003C\/em\u003E using RNA-seq and mass spectrometry approaches, and will integrate with their half-life dynamics for thousands of genes (work in progress). These studies provide a quantitative analysis of the cellular abundance and turnover dynamics of mRNA and protein and help us understand the underlying principles of gene expression control.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe seminar will be hosted at \u003Ca href=\u0022https:\/\/bluejeans.com\/558060212\u0022\u003Ethis bluejeans link\u003C\/a\u003E.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003E\u003Cem\u003EQBioS 4th year student Mengshi Zhang (homeschool of biological sciences), advised by Marvin Whiteley, will present \u0026quot;Quantification of mRNA and protein and integration with their half-life dynamics in Aggregatibacter actinomycetemcomitans\u0026quot;\u003C\/em\u003E\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"QBioS Student Seminar Series - Mengshi Zhang"}],"uid":"35673","created_gmt":"2021-02-24 00:30:16","changed_gmt":"2021-02-24 00:30:16","author":"dmuratore3","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2021-02-25T16:00:00-05:00","event_time_end":"2021-02-25T17:00:00-05:00","event_time_end_last":"2021-02-25T17:00:00-05:00","gmt_time_start":"2021-02-25 21:00:00","gmt_time_end":"2021-02-25 22:00:00","gmt_time_end_last":"2021-02-25 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"644610":{"id":"644610","type":"image","title":"02_25_qbios_seminar_flyer","body":null,"created":"1614126211","gmt_created":"2021-02-24 00:23:31","changed":"1614126211","gmt_changed":"2021-02-24 00:23:31","alt":"","file":{"fid":"244747","name":"02_25_announcement.png","image_path":"\/sites\/default\/files\/images\/02_25_announcement.png","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/02_25_announcement.png","mime":"image\/png","size":122049,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/02_25_announcement.png?itok=xXYqjzrB"}}},"media_ids":["644610"],"groups":[{"id":"562111","name":"QBioS"}],"categories":[],"keywords":[{"id":"138191","name":"go-qbios"},{"id":"147941","name":"go_qbios"},{"id":"168667","name":"QBioS"},{"id":"182721","name":"Qbios seminar"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"177814","name":"Postdoc"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EBluejeans link for the seminar is available\u0026nbsp;\u003Ca href=\u0022https:\/\/bluejeans.com\/558060212\u0022\u003Ehere\u003C\/a\u003E.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EContact Daniel Muratore (\u003Ca href=\u0022https:\/\/qbios.gatech.edu\/people\/Daniel%20Muratore\u0022\u003Ehere\u003C\/a\u003E) for questions.\u0026nbsp;\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}