{"655398":{"#nid":"655398","#data":{"type":"event","title":"MS Defense by Jessica Pfliger","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMaster of Science in Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ein the\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EJessica Pfliger\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWill defend her thesis\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026ldquo;CHARACTERIZATION OF A CHROMATINIZED HYDROGEN PEROXIDE BIOSENSOR IN CANCER CELLS\u0026rdquo;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E14, February 2022\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;1:00pm\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Ca href=\u0022https:\/\/bluejeans.com\/126058378\u0022\u003Ehttps:\/\/bluejeans.com\/126058378\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Yuhong Fan\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Alfred Merrill\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;Dr. Hengbin Wang\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Biochemistry and Molecular Genetics\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EUniversity of Alabama at Birmingham\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHydrogen Peroxide (H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E) is an important eukaryotic signaling molecule regulating cellular processes. As one of the most abundant reactive oxidative species (ROS), H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E can cause oxidative stress and cytotoxicity at elevated concentrations leading to DNA damage and programmed cell death. Despite the recent advancements in modulating ROS responses for potential cancer therapies, little is known about nuclear ROS temporal emergence and dynamics. To investigate the role of nuclear ROS and H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E in chromatin, we established a genetically engineered chromatin-targeted biosensor for H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E, H2B-HyPer, by fusion of HyPer, a specific H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E biosensor, with core histone H2B. In this thesis, I utilized fluorescent microscopy and fluorescent recovery after photobleaching (FRAP) to study the H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E dynamics within the chromatin of HCT116 colon cancer cells containing genetically integrated H2B-HyPer. I demonstrate that H2B-HyPer is localized in the nucleus of HCT116\/H2B-HyPer cells with an average residence time and mobile fraction of 9.14 minutes and 20.59%, respectively, comparable to the core histones in chromatin. I then show that H2B-HyPer is sensitive to changes in H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E levels post addition of H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E or DTT in culture medium. Further analysis of H2B-HyPer kinetics revealed a rapid increase and recovery of H2B-HyPer signal intensity in HCT116\/H2B-HyPer cells upon treatment with and the removal of H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E. Altogether, these studies establish H2B-HyPer as an effective biosensor for real-time spatio-temporal tracking of chromatin-proximal H\u003Csub\u003E2\u003C\/sub\u003EO\u003Csub\u003E2\u003C\/sub\u003E dynamics.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"  \u201cCHARACTERIZATION OF A CHROMATINIZED HYDROGEN PEROXIDE BIOSENSOR IN CANCER CELLS\u201d"}],"uid":"27707","created_gmt":"2022-02-11 15:53:03","changed_gmt":"2022-02-11 15:53:03","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-02-14T13:00:00-05:00","event_time_end":"2022-02-14T15:00:00-05:00","event_time_end_last":"2022-02-14T15:00:00-05:00","gmt_time_start":"2022-02-14 18:00:00","gmt_time_end":"2022-02-14 20:00:00","gmt_time_end_last":"2022-02-14 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"111531","name":"ms defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}