<![CDATA[test]]> 27213 test

]]> Teri Nagel 1 1274355880 2010-05-20 11:44:40 1475891489 2016-10-08 01:51:29 0 0 event 2010-02-18T08:00:00-05:00 2010-02-18T09:00:00-05:00 2010-02-18T09:00:00-05:00 2010-02-18 13:00:00 2010-02-18 14:00:00 2010-02-18 14:00:00 2010-02-18T08:00:00-05:00 2010-02-18T09:00:00-05:00 America/New_York America/New_York datetime 2010-02-18 08:00:00 2010-02-18 09:00:00 America/New_York America/New_York datetime <![CDATA[]]> Corissa Jones
Music Department, College of Architecture
Contact Corissa Jones
404-894-8949]]> <![CDATA[Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces]]> 27195 ABSTRACT
Using an ultrasensitive force probe and basic statistical principles, we have developed a novel method to assay both the kinetic rates at which adhesive ligands dissociate from cell receptors and the rates at which the receptors separate from the cytoskeleton in the course of single molecule pulling experiments. Using results from probe tests of ICAM-1 bonds to L 2 integrin on leukocytes, I will show that cell signaling not only regulates lifetimes and strengths of the outer-ligand adhesive bond but also lifetimes and strengths of cohesive integrin anchoring to the cell cytostructure. Whether triggered from outside or inside the cell, the results indicate that the mechanical linkage between integrin tail domains and the cytoskeleton is a key effector of integrin activation in cell signaling (e.g. analogous to the “mechanical transmission” in an automobile).

 

]]> Colly Mitchell 1 1298974491 2011-03-01 10:14:51 1475891666 2016-10-08 01:54:26 0 0 event BIOE Seminar Series

Evan Evans, PhD - Professor, Department of Physics, Boston University

"Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces"

]]> 2011-03-29T15:00:00-04:00 2011-03-29T16:00:00-04:00 2011-03-29T16:00:00-04:00 2011-03-29 19:00:00 2011-03-29 20:00:00 2011-03-29 20:00:00 2011-03-29T15:00:00-04:00 2011-03-29T16:00:00-04:00 America/New_York America/New_York datetime 2011-03-29 03:00:00 2011-03-29 04:00:00 America/New_York America/New_York datetime <![CDATA[]]> James Godard

Faculty host:  Cheng Zhu

]]> <![CDATA[Evans Profile]]> <![CDATA[Molecular Engineering and Live Cell Imaging for Studying Cell-Environment Interactions]]> 27195 Abstract:

Signaling molecules and their activities are well coordinated inspace and time to regulate cellular functions in response to mechanicaland chemical microenvironment. Based on fluorescent resonance energytransfer (FRET), we have developed several genetically encodedbiosensors for detecting the spatiotemporal activities of signalingmolecules, including Src, Rac1, MT1-MMP, and Calcium. A Rac biosensorrevealed that the Rac activity in cells constrained on micropatternedextracellular-matrix surface is polarized with higher activityconcentrated at the leading edge of migrating cells upon PDGFstimulation, whereas Src activities in these cells displayed globalactivation patterns without obvious polarity. Our calcium biosensoralso allowed the revelation that there is a spontaneous Ca2+oscillation in human mesenchymal stem cells (HMSCs) both inside thecytoplasm and endoplasmic reticulum (ER). The substrate stiffness whereHMSCs are seeded can significantly affect this Ca2+ oscillation, in afashion dependent on the RhoA signaling pathway. We have furtherdeveloped a FAK FRET biosensor and targeted it into lipid rafts ornon-rafts of plasma membrane by lipid modifications. Upon cell adhesionon extracellular matrix proteins or stimulation by platelet-derivedgrowth factor, the raft-targeting FAK biosensor showed a surprisinglystronger FRET response than that at non-rafts, suggesting that the FAKactivation mainly occurs at lipid rafts. Further experiments revealedthat the PDGF-induced FAK activation at rafts is mediated by the kinaseactivity of Src, whereas FAK activation induced by adhesion isindependent of, and in fact essential for the Src activation. Theseresults suggest that FAK is activated at rafts with distinct activationmechanisms in response to different physiological stimuli. In summary,our novel FRET biosensors in combination with tools innano-biotechnology and bio-photonics have made it possible to monitorkey signaling cascades in live cells with subcellular and dynamiccharacterization when cells interact with their physical/chemicalmicroenvironment.

]]> Colly Mitchell 1 1297421339 2011-02-11 10:48:59 1475891645 2016-10-08 01:54:05 0 0 event Bioengineering Seminar Series

Yingxiao Wang, PhD - University of Illinois, Urbana-Champaign

Molecular Engineering and Live Cell Imaging for Studying Cell-Environment Interactions

]]> 2011-03-01T10:00:00-05:00 2011-03-01T11:00:00-05:00 2011-03-01T11:00:00-05:00 2011-03-01 15:00:00 2011-03-01 16:00:00 2011-03-01 16:00:00 2011-03-01T10:00:00-05:00 2011-03-01T11:00:00-05:00 America/New_York America/New_York datetime 2011-03-01 10:00:00 2011-03-01 11:00:00 America/New_York America/New_York datetime <![CDATA[]]> Faculty Host: Andres Garcia 404-894-9384

]]> <![CDATA[Wang Lab]]>