{"659956":{"#nid":"659956","#data":{"type":"event","title":"PhD Defense by Siu Lung (Michael) Ng","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn the\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ESiu Lung (Michael) Ng\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWill defend his dissertation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EEvolution of Bacterial Type VI Secretion System Regulation and Defense\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E18\u003Csup\u003Eth\u003C\/sup\u003E August 2022\u003C\/p\u003E\r\n\r\n\u003Cp\u003E2 p.m.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPrice Gilbert Library 4222 or \u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/7036781501\u0022\u003Ehttps:\/\/gatech.zoom.us\/j\/7036781501\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBrian Hammer, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMarvin Whiteley, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWilliam Ratcliff, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EFrank Stewart, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Microbiology and Immunology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMontana State University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPeter Yunker, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Physics\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EABSTRACT:\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBacteria in nature live in communities with other organisms where resources and space are typically lacking. To compete, bacteria have evolved strategies to increase fitness by eliminating unwanted competitors with secreted goods such as traditional, diffusible antibiotics. A more recently described mechanism of antagonism is a contact-dependent \u0026rdquo;nanoharpoon\u0026rdquo;, the type VI secretion system (T6), which kills neighboring \u0026rdquo;target\u0026rdquo; cells by directly injecting lethal toxins.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe broadly distributed T6 weapon plays an important role in competition and the pathogenicity of many Gram-negative bacteria including \u003Cem\u003EVibrio cholerae\u003C\/em\u003E, with prior studies demonstrating a role for the \u003Cem\u003EV. cholerae\u003C\/em\u003E T6 in host colonization and infection. The majority of our understanding of this apparatus in \u003Cem\u003EV. cholerae\u003C\/em\u003E is based on studies with isolates that contribute to disease. The current regulatory model of T6 control describes a requirement of one of the two transcription factors: QstR, which is induced by external chemical cues, or TfoY, which is activated by intracellular signals. By contrast, our knowledge of the T6 of \u003Cem\u003EV. cholerae\u003C\/em\u003E strains from non-human sources is more limited. So too, the structure of the T6 apparatus, the toxic effector proteins delivered by it, and their corresponding immunity proteins have garnered much attention. Yet we know little about how microbes may protect\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ethemselves against T6 attack.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn my dissertation, I describe two major contributions to the field. First, I determined that QstR and TfoY are either dispensable or only play a minor role in \u003Cem\u003EV. cholerae\u003C\/em\u003E strains isolated from nonhuman sources, which instead display constitutive T6 activity in laboratory conditions. To begin to determine the mechanism responsible for regulatory differences between human and nonhuman strains, I successfully mapped the promoter region of the major T6 gene cluster and identified a single-nucleotide polymorphism (SNP) in the DNA sequence upstream of the promoter that affects the regulation of T6. Human-derived, QstR-regulated strains encode a guanine (G) at this position, while environmental strains carry a thymine (T), with varying contributions of TfoY. These results are consistent with a \u0026rdquo;pathoadaptive\u0026rdquo; theory that \u003Cem\u003EV. cholerae\u003C\/em\u003E dampens the T6 regulation during infection and displays a constitutive T6 activity in natural environments. Second, I participated in the discovery that target cells have two major mechanisms to resist T6 attacks. Using experimental evolution, I help uncover that \u003Cem\u003EEscherichia coli\u003C\/em\u003E becomes less susceptible to T6 antagonism following several hundred generations of repeated T6-mediated competition. We identified three genes that contribute to the T6 resistance: \u003Cem\u003EapaH\u003C\/em\u003E disruption, a specific \u003Cem\u003EyjeP\u003C\/em\u003E missense mutation, and \u003Cem\u003EyejM\u003C\/em\u003E mutations that result in C-terminus disruptions. The T6 resistance is greater when \u003Cem\u003EE. coli\u003C\/em\u003E carries both the \u003Cem\u003EyjeP\u003C\/em\u003E and \u003Cem\u003EyejM\u003C\/em\u003E mutations. These mutations however are pleiotropic, reducing growth rate and causing other collateral effects, supporting a hypothesis that evolution of T6 resistance in natural communities is likely constrained by fitness effects. This dissertation provides insight into how T6 aggression and resistance are two facets of an evolutionary arms race: with killer cells evolving strategies for antagonism that provide selective pressure favoring target cells with T6 resistance.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Evolution of Bacterial Type VI Secretion System Regulation and Defense "}],"uid":"27707","created_gmt":"2022-08-05 14:35:30","changed_gmt":"2022-08-05 14:35:30","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-08-18T15:00:00-04:00","event_time_end":"2022-08-18T17:00:00-04:00","event_time_end_last":"2022-08-18T17:00:00-04:00","gmt_time_start":"2022-08-18 19:00:00","gmt_time_end":"2022-08-18 21:00:00","gmt_time_end_last":"2022-08-18 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}