{"68630":{"#nid":"68630","#data":{"type":"event","title":"IBB Breakfast Club Seminar Series","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u0022Automated Whole-cell\nPatch\nClamp\nElectrophysiology\nof\nNeurons\nin\nvivo\u0022\u003Cbr \/\u003E\u003Cbr \/\u003ECraig Forest, PhD\u003C\/strong\u003E - Assistant Professor, George W. Woodruff School of Mechanical Engineering\u003C\/p\u003E\u003Cp\u003EABSTRACT: \u0026nbsp;Whole-cell patch clamp electrophysiology of neurons in vivo enables the recording of electrical events in cells with great\u0026nbsp;precision, and supports a wide diversity of cellular morphological and\u0026nbsp;molecular analysis experiments. \u0026nbsp;However, high levels of skill are\u0026nbsp;required in order to perform in vivo patching, and the process is\u0026nbsp;time-consuming and painstaking. \u0026nbsp;An automated in vivo patching robot\u0026nbsp;would not only empower a great number of neuroscientists to perform\u0026nbsp;such experiments, but would also open up fundamentally new kinds of\u0026nbsp;experiment enabled by the resultant high throughput. \u0026nbsp;We discovered\u0026nbsp;that in vivo blind whole cell patch clamp electrophysiology could be\u0026nbsp;implemented as a straightforward algorithm, and developed an automated robotic system capable of performing this algorithm. \u0026nbsp;We validated the performance of our robot in both the cortex and hippocampus of anesthetized mice. \u0026nbsp;Our robot achieves yields, cell recording qualities, and operational speeds that are comparable to, or exceed, those of experienced human investigators, and is simple and\u0026nbsp;inexpensive to implement.\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EThe IBB Breakfast Club seminar series was started with the spirit of the Institute\u0027s interdisciplinary mission in mind. The goal of the seminar series is to highlight research taking place throughout the institute to enable the IBB community to further collaborative opportunities and interdisciplinary research. Faculty are often asked to speak at other universities and conferences, but rarely present at their home institution, this seminar series is an attempt to close that gap. The IBB Breakfast Club is open to anyone in the bio-community.\u003Cbr \/\u003E\u003Cbr \/\u003EContinental breakfast will be served.\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EIBB Breakfast Club Seminar Series\u003C\/p\u003E\u003Cp\u003ECraig Forest, PhD - Assistant Professor, School of Mechanical Engineering\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Automated Whole-cell Patch Clamp Electrophysiology of Neurons in vivo\u0022"}],"uid":"27195","created_gmt":"2011-06-29 09:50:40","changed_gmt":"2016-10-08 01:55:08","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-10-11T09:30:00-04:00","event_time_end":"2011-10-11T10:30:00-04:00","event_time_end_last":"2011-10-11T10:30:00-04:00","gmt_time_start":"2011-10-11 13:30:00","gmt_time_end":"2011-10-11 14:30:00","gmt_time_end_last":"2011-10-11 14:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"68626":{"id":"68626","type":"image","title":"Craig Forest, PhD - Assistant Professor, Mechanical Engineering","body":null,"created":"1449177185","gmt_created":"2015-12-03 21:13:05","changed":"1475894597","gmt_changed":"2016-10-08 02:43:17","alt":"Craig Forest, PhD - Assistant Professor, Mechanical Engineering","file":{"fid":"192621","name":"forest.jpg","image_path":"\/sites\/default\/files\/images\/forest_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/forest_0.jpg","mime":"image\/jpeg","size":6910,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/forest_0.jpg?itok=7iDCnO5X"}},"70917":{"id":"70917","type":"image","title":"Breakfast Club Seminar Series","body":null,"created":"1449177328","gmt_created":"2015-12-03 21:15:28","changed":"1475894625","gmt_changed":"2016-10-08 02:43:45","alt":"Breakfast Club Seminar Series","file":{"fid":"193471","name":"logobldg_graphic.png","image_path":"\/sites\/default\/files\/images\/logobldg_graphic_0.png","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/logobldg_graphic_0.png","mime":"image\/png","size":241588,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/logobldg_graphic_0.png?itok=x84xf3Mz"}}},"media_ids":["68626","70917"],"related_links":[{"url":"internal:\/Craig Forest, PhD - Assistant Professor, School of Mechanical Engineering","title":"Forest lab"},{"url":"http:\/\/ibb.gatech.edu\/breakfast-club","title":"Breakfast Club series schedule"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"11879","name":"BK Club"},{"id":"12333","name":"Craig Forest"},{"id":"248","name":"IBB"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:colly.mitchell@ibb.gatech.edu\u0022\u003EColly Mitchell\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}