{"69114":{"#nid":"69114","#data":{"type":"event","title":"IBB Seminar","body":[{"value":"\u003Cp\u003E\u003Cem\u003E\u003Cstrong\u003E\u0022Fabrication of 3D Tissue Chips Using a Layer-by-Layer Technique\u0022\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003E\u003Cbr \/\u003EMitsuru Akashi, PhD\u003C\/strong\u003E - Osaka University\u003Cbr \/\u003E\u003C\/em\u003E\u003Cem\u003E\u003Cbr \/\u003EAbstract\u003Cbr \/\u003E\u003C\/em\u003EA Layer-by-Layer (LbL) assmebly is an appropriate method\nto fabricate nanometer-sized films on a substrate through alternate immersion\ninto interactive polymer solutions. The LbL hierarchic construction of\nfilms can be achieved by introducing heterogeneous polymers, resulting in novel\npolymer blend systems. We focused\non the fabrication of stereocomplex LbL films by van der Waals interactions and\nthe construction of biocompatible hollow capsules for drug delivery system by\nLbL assembly. The multilayered films with tuneable physic-chemical\nproperties prepared by LbL assembly have great potential for varied\napplications.\u003C\/p\u003E\n\n\u003Cp\u003EThe\ncreation of artificial three-dimensional (3D) tissues possessing a similar\nstructure and functions as natural tissue is a key challenge for implantable\ntissues in tissue engineering, and for model tissues in pharmaceutical assay.\nRecently, we demonstrated a simple 3D-cell manipulation technique for the\nconstruction of hierarchically layered structures, which possess a precisely\ncontrolled layer number and type of cells, by the direct fabrication of a fibronectin\n(FN)-gelatin (G) nanofilm like an ECM on the surface of each cell layer. Since\nthe FN-G nanofilms with over 6 nm thick acted as a scaffold for the cell\nadhesion of the second layer, we successfully developed hierarchical cellular\nmultilayers composed of various type of cells, such as human primary\nfibroblasts, mouse C2C12 myoblasts, rat primary cardiac myocytes, and human\nprimary smooth muscle cells, even 10 layers In addition, blood vessel model\ntissues with varied layer numbers composed of human smooth muscle cell (UASMC)\nand human endothelial cell (HUVEC) were also fabricated by this technique.\nThese hierarchical structures would be useful as a 3D-layered model tissue for\ntissue engineering and pharmaceutical assay.\u003C\/p\u003E\n\n\u003Cp\u003E\u003Cstrong\u003EReference: \u003C\/strong\u003EMichiya Matsusaki, Koji Kadowaki, Yoshio Nakahara,\nMitsuru Akashi, \u201cFabrication of Cellular Multilayers with Nanometer-Sized\nExtracellular Matrix Films\u201d\u003Cstrong\u003E\u003Cem\u003E, Angew. Chem. Int. Ed.,\u003C\/em\u003E\u003C\/strong\u003E \u003Cstrong\u003E46\u003C\/strong\u003E,\n4689-4692 (2007).\u003Cstrong\u003E\u003C\/strong\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EIBB Special Seminar\u003C\/p\u003E\u003Cp\u003E\u0022Fabrication of 3D Tissue Chips Using a Layer-by-Layer Technique\u0022\u003C\/p\u003E\u003Cp\u003EMitsuru Akashi, PhD - Osaka University\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Fabrication of 3D Tissue Chips Using a Layer-by-Layer Technique\u0022 - Mitsuru Akashi, PhD - Osaka University"}],"uid":"27195","created_gmt":"2011-08-02 08:15:22","changed_gmt":"2016-10-08 01:55:18","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-08-30T12:00:00-04:00","event_time_end":"2011-08-30T13:00:00-04:00","event_time_end_last":"2011-08-30T13:00:00-04:00","gmt_time_start":"2011-08-30 16:00:00","gmt_time_end":"2011-08-30 17:00:00","gmt_time_end_last":"2011-08-30 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"69116":{"id":"69116","type":"image","title":"Mitsuru Akashi, PhD - Osaka University","body":null,"created":"1449177228","gmt_created":"2015-12-03 21:13:48","changed":"1475894604","gmt_changed":"2016-10-08 02:43:24","alt":"Mitsuru Akashi, PhD - Osaka University","file":{"fid":"192707","name":"makashi.jpg","image_path":"\/sites\/default\/files\/images\/makashi_0.jpg","image_full_path":"http:\/\/www.tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/makashi_0.jpg","mime":"image\/jpeg","size":15241,"path_740":"http:\/\/www.tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/makashi_0.jpg?itok=hoYTxw6d"}}},"media_ids":["69116"],"related_links":[{"url":"http:\/\/www.chem.eng.osaka-u.ac.jp\/~akashi-lab\/Home_e\/index.html","title":"Akashi lab"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"4912","name":"Andrew Lyon"},{"id":"13852","name":"Fabrication of 3D Tissue Chips Layer Technique"},{"id":"248","name":"IBB"},{"id":"5168","name":"IBB Seminar"},{"id":"13851","name":"Mitsuru Akashi"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EFaculty host: \u0026nbsp;Andrew Lyon, PhD\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}