{"92241":{"#nid":"92241","#data":{"type":"event","title":"(09-0903) Prof. Aaron Wheeler, University of Toronto","body":[{"value":"\u003Cp\u003EProf. Aaron Wheeler, University of Toronto\n\u003C\/p\u003E\n\u003Cp\u003EHybrid Microfluidics for Integrated Proteomics\n\u003C\/p\u003E\n\u003Cp\u003EA common theme in chemical analyses (across a broad range of applications) is the requirement of sample processing and separations. For example, in shotgun proteomics, samples are subjected to a rigorous processing regimen (including numerous pipetting steps and reagents, as well as mixing, heating, and centrifugation) followed by two-dimensional chromatography and tandem mass spectrometry. The sample processing required for shotgun proteomics is time-consuming and tedious, and more problematically, the methodologies are not standardized, resulting in pervasive lack of reproducibility from lab to lab. In response to this challenge, we are developing a miniaturized lab-on-a-chip platform integrating and automating sample processing and separations\/mass spectrometry for shotgun proteomics. In this platform, separations are implemented in the common format of microchannels, while sample processing is achieved using \u00e2\u0080\u009cdigital microfluidics,\u00e2\u0080\u009d a relatively new technique in which droplets of reagents are manipulated electromechanically on an open surface. In digital microfluidics, each droplet can be individually addressed, making it a good match for implementing precise multistep reactions. Here, we present a hybrid microfluidic platform linking digital microfluidics for on-chip protein precipitation, reduction, alkylation, and enzymatic digestion and microchannels for chromatography. We anticipate that future generations of this technology will be capable of analyzing many samples in parallel, and we speculate that tools such as these will be useful for standardizing chemical analyses for shotgun proteomics and other applications.\n\u003C\/p\u003E\n\u003Cp\u003EFor more information contact \u003Ca href=\u0022mailto:facundo.fernandez@chemistry.gatech.edu\u0022\u003EProf. Facundo Fernandez\u003C\/a\u003E (404-385-4432).\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"Prof. Aaron Wheeler, University of Toronto\n\nHybrid Microfluidics for Integrated Proteomics","format":"limited_html"}],"field_summary_sentence":[{"value":"Prof. Aaron Wheeler, University of Toronto"}],"uid":"27275","created_gmt":"2009-02-27 01:00:00","changed_gmt":"2016-10-08 01:57:11","author":"Shirley Tomes","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2009-09-03T16:00:00-04:00","event_time_end":"2009-09-03T17:00:00-04:00","event_time_end_last":"2009-09-03T17:00:00-04:00","gmt_time_start":"2009-09-03 20:00:00","gmt_time_end":"2009-09-03 21:00:00","gmt_time_end_last":"2009-09-03 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/www.chem.utoronto.ca\/peoples\/faculty_profile.php?id=84","title":"Prof. Aaron Wheeler, University of Toronto"}],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[{"id":"89","name":"chemistry"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003EShirley Tomes\u003C\/strong\u003E\u003Cbr \/\u003EChemistry \u0026amp; Biochemistry\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=st81\u0022\u003EContact Shirley Tomes\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-0591\u003C\/strong\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}